@article {3136442,
	title = {Quantitative methods for the analysis of CFTR transcripts/splicing variants},
	journal = {J Cyst Fibros},
	volume = {3 Suppl 2},
	year = {2004},
	month = {2004 Aug},
	pages = {17-23},
	abstract = {In cystic fibrosis (CF), transcript analysis and quantification are important for diagnosis, prognosis and also as surrogate markers for some therapies including gene therapy. Classical RNA-based methods require significant expression levels in target samples for appropriate analysis, thus PCR-based methods are evolving towards reliable quantification. Various protocols for the quantitative analysis of CFTR transcripts (including those resulting from splicing variants) are described and discussed here.},
	keywords = {Cystic Fibrosis, Cystic Fibrosis Transmembrane Conductance Regulator, Genetic Techniques, Humans, Reverse Transcriptase Polymerase Chain Reaction, Transcription, Genetic},
	issn = {1569-1993},
	doi = {10.1016/j.jcf.2004.05.047},
	author = {Amaral, Margarida D and Clarke, Luka A and Ramalho, Anabela S and Beck, Sebastian and Broackes-Carter, Fiona and Rowntree, Rebecca and Mouchel, Nathalie and Williams, Sarah H and Harris, Ann and Tzetis, Maria and Steiner, Bernhard and Sanz, Javier and Gallati, Sabina and Nissim-Rafinifa, Malka and Kerem, Batsheva and Hefferon, Timothy and Cutting, Garry R and Goina, Elisa and Pagani, Franco}
}
